What is CytReg_v2?
CytReg_v2 is an extension of CytReg, a manually curated database of published interactions between transcription factors (TFs) and cytokine genes determined from functional assays (e.g. reporter assays), in vivo binding (e.g. chromatin immunoprecipitation), and in vitro DNA binding (e.g. EMSA). CytReg_v2 includes additional published interactions and interactions determined from high throughput enhanced yeast one-hybrid (eY1H) assays.
Interactions between TFs and cytokine genes of interest can be searched in the Search page, and filtered by species (human or mouse), evidence (ChIP, eY1H, binding, or functional), and TF expression levels in immune cells. The selected interactions can then be visualized as a table with links to Uniprot for each TF and cytokine gene, and links to publications reporting the interaction. Additionally, the data can be visualized as a network connecting TFs and target cytokine genes or downloaded as a csv file.
How to cite CytReg
Santoso CS, Li Z, Lal S, Yuan S, Gan KA, Agosto LM, Liu X, Carrasco Pro S, Sewell JA, Henderson A, Maninjay AK, Fuxman Bass JI. Comprehensive mapping of the human cytokine gene regulatory network. Nucleic Acids Res. 2020 2020 Dec 2;48(21):12055-12073.
Contact information
Juan Fuxman Bass (email : fuxman@bu.edu) www.fuxmanlab.com
Funding
This work was supported by the National Institutes of Health [grant numbers R00 GM114296 and R35 GM128625 to J.I.F.B., R01 AI138960 and R01 AI122842 to A.H.J., 5T32HL007501-34 to J.A.S.]; the amfAR Mathilde Krim Fellowship [109263-59-RKRL to L.M.A] and Boston University’s Undergraduate Research Opportunities Program and New England Biolabs’ Summer Undergraduate Research Award [to S.Y].